Abstract：Bacterial infection seriously affects human health, and peroxidase-like (POD-like) nanozymes open up promising avenues for innovative antibacterial strategies beyond traditional antibiotics. In this study, we developed a molybdenum disulfide quantum dot-decorated mesoporous polydopamine nanoparticle (MPM) with highly efficient POD-like catalytic activity, H2O2 production and GSH responsive properties. Molybdenum disulfide quantum dots act as POD-like nanozymes, while mesoporous polydopamine act as a H2O2 generator, GSH consumption agent and nanocarrier. Specifically, MPM could promote the decomposition of overexpressed hydrogen peroxide (H2O2) within the bacterial infection microenvironment into hydroxyl radicals (·OH) without the need for external activation, thereby disrupting bacterial structure and function, leading to bacterial death. Additionally, MPM generates H2O2 and consumes GSH, ensuring the persistence and efficacy of ROS-based antibacterial activity. In vitro antibacterial experiments showed that E. coli and S. aureus was inhibited by MPM at rate of 96.3% and 100%, respectively. The antibiofilm experiment further demonstrated that MPM maintained high POD activity and can effectively destroy and inhibit the formation of bacterial biofilm, offering a new strategy for developing antibiotic-free antibacterial methods. In vivo experiments showed that MPM could effectively eliminate bacteria in wound tissues of mice and had a good therapeutic effect on infected wounds. Moreover, MPM exhibits good biosafety, indicating that it has ideal application potential as a highly effective and safe antibacterial agent.